Forty-two Premier League players and staff tested positive for Covid-19 in the past week.
This is the most recorded in the league over a seven-day period.
It is the highest number since 40 cases were reported in January.
That week, 2,295 tests were carried out, while 3,805 tests were done in the latest round from 6-12 December.
Brighton, Tottenham, Leicester, Manchester United, Aston Villa and Norwich have all confirmed cases, with United’s game at Brentford postponed.
Sunday’s Brighton v Tottenham fixture was also postponed following a Covid-19 outbreak at Spurs.
Previously, the most positive tests returned in a week this season was 16 from 16-22 August, when there were 3,060 tests.
The latest report of 42 results is an increase of 30 on the 12 positives reported from 3,154 tests from 29 November – 5 December.
On Thursday, following the introduction of new coronavirus rules in England, the Premier League told clubs to return to emergency measures, including social distancing and wearing masks.
A league statement on Monday said the frequency of lateral flow and PCR tests for players and staff would increase.
The statement added: “We will continue to work closely with the government, local authorities and supporter groups, while being responsive to any future changes to national or local guidance.”
Brighton chief executive Paul Barber told BBC Sport clubs are “now moving to testing three times a week” for players and the staff that work “in the bubble” to try and limit the number of infections that are spread from one player to another.
And from Wednesday, fans will need to show proof of double vaccination or a negative test to attend sporting events with crowds of more than 10,000 people in England. This includes Premier League games.
